Crowley, D.J., research program has focused on elucidating mechanisms and regulation of DNA repair and DNA damage tolerance in the extremely halophilic archaeon, Halobacterium sp. NRC-1 and the moderately halophilic Haloferax volcanii. Projects of ongoing interest include modification and optimization of assay systems to study transcription-coupled repair of ultraviolet light-induced DNA lesions in Halobacterium sp. NRC-1. Also targeted knockouts of putative repair genes inHalobacterium for purposes of functional characterization. Twenty-five undergraduate students have participated in this research at Assumption College and Mercer University. Recent collaborators have included Dr. Shirley McCready at Oxford-Brookes University, Oxford, UK and Dr. Justin Courcelle at Portland State University, Portland, OR.
Dissertation Research, Stanford University, 1994-1999
Research in the laboratory of Dr. Philip Hanawalt. DNA damage inducible responses and mechanisms of DNA repair inEscherichia coli. Major focus on the genetic control of nucleotide excision repair genes and the effects of their regulation on the efficiency of repair of ultraviolet light induced DNA lesions. Additional studies characterizing modes of DNA repair in Haloferax volcanii.
Undergraduate Honors Thesis, Univ. of Massachusetts Medical Center, 1991-1993
Research in the laboratory of Dr. Michael Volkert. Development of an assay to measure transcription-coupled DNA repair of ultraviolet light irradiated phage lambda in its host, Escherichia coli. Also contributed to elucidating modes of DNA repair and mutagenesis in aerobic and anaerobically grown Escherichia coli treated with alkylating or oxidizing chemicals.
Undergraduate Research, College of the Holy Cross, 1991-1992
Research in the laboratory of Dr. George Hoffmann. Investigated the effect of monofunctional and bifunctional alkylating agents on the rates of mutation and recombination in Salmonella typhimurium.